Construction and Rescue of a Minigenome System for Lentogenic D58 Strain of Avian orthoavulavirus 1 (Newcastle Disease Virus)

Background: In reverse genetics studies, minigenomes of negative sense RNA viruses are used to model the process virus replication and evaluate rescue system in course construction recombinant virus. current study, a minigenome (MG) for lentogenic D58 vaccine strain Avian orthoavulavirus 1 (AOAV-1) (Newcastle disease virus) was developed establish genetic this strain. Methods: The constructed by assembling trailer leader regions AOAV-1 flanking reporter gene, Aequorea coerulescens green fluorescence protein (AcGFP) modified transcription vector. Construction Minigenome done using simple restriction free technique-Splicing Overlap Extension PCR. evaluated gene expression transfection T7 polymerase expressing BSR/T7 cell line. Further, packaging ability were also analyzed. Result: confirmed cells which simulates verifies system. addition, proves applicability cloning Splicing (SOE) PCR precise minigenome. can be as potential tool development marker vaccine.